Recovery of hematopoietic colony-forming cells in irradiated mice pretreated with interleukin 1 (IL-1)

Data in this report determined the effect of a single injection of recombinant interleukin 1 alpha (rIL-1) prior to irradiation of B6D2F1 mice on the recovery of colony-forming cells (CFC) at early and late times after sublethal and lethal doses of radiation. Injection of rIL-1 promoted an earlier r...

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Bibliographic Details
Published inExperimental hematology Vol. 16; no. 9; p. 752
Main Authors Schwartz, G N, Neta, R, Vigneulle, R M, Patchen, M L, MacVittie, T J
Format Journal Article
LanguageEnglish
Published Netherlands 01.10.1988
Subjects
Animals
Colony-Forming Units Assay
Female
Hematopoiesis - radiation effects
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - radiation effects
Injections, Intraperitoneal
Interleukin-1 - therapeutic use
Mice
Mortality
Radiation Chimera
Recombinant Proteins - therapeutic use
Sodium Chloride - administration & dosage
Time Factors
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Summary:Data in this report determined the effect of a single injection of recombinant interleukin 1 alpha (rIL-1) prior to irradiation of B6D2F1 mice on the recovery of colony-forming cells (CFC) at early and late times after sublethal and lethal doses of radiation. Injection of rIL-1 promoted an earlier recovery of mature cells in the blood and CFC in the bone marrow and spleen. For example, 8 days after 6.5 Gy irradiation, the number of CFU-E (colony-forming units-erythroid), BFU-E (burst-forming units-erythroid), and GM-CFC (granulocyte-macrophage colony-forming cells) per femur was approximately 1.5-fold higher in rIL-1-injected mice than in saline-injected mice. Also, 5, 9, and 12 days after irradiation, the number of both day 8 and day 12 CFU-S (colony-forming units-spleen) was almost twofold greater in bone marrow from rIL-1-injected mice. The earlier recovery of CFU-S in rIL-1-injected mice was not associated with an increase in the number of CFU-S that survived immediately after irradiation. Also, 7 months after irradiation, the number of CFU-S per femur of both saline- and rIL-1-injected mice was still less than 50% of normal values. Data in this report demonstrate that a single injection of rIL-1 prior to irradiation accelerates early hematopoietic recovery in irradiated mice, but does not prevent expression of radiation-induced frontend damage or long-term damage to hematopoietic tissues.
ISSN:0301-472X