Binding of iodinated recombinant human GM-CSF to the blast cells of acute myeloblastic leukemia

Granulocyte/macrophage-colony-stimulating factor (GM-CSF) is an effective growth factor for the blasts of acute myeloblastic leukemia (AML). Radioiodinated Chinese hamster ovary (CHO)-cell derived GM-CSF was prepared using Bolton-Hunter reagent to label free amino groups on the protein. Normal human...

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Bibliographic Details
Published inLeukemia Vol. 2; no. 4; p. 211
Main Authors Kelleher, C A, Wong, G G, Clark, S C, Schendel, P F, Minden, M D, McCulloch, E A
Format Journal Article
LanguageEnglish
Published England 01.04.1988
Subjects
Colony-Stimulating Factors - metabolism
Granulocyte-Macrophage Colony-Stimulating Factor
Growth Substances - metabolism
Humans
Iodine Radioisotopes
Leukemia, Myeloid, Acute - metabolism
Neoplastic Stem Cells - metabolism
Neutrophils - metabolism
Receptors, Cell Surface - analysis
Receptors, Colony-Stimulating Factor
Recombinant Proteins - metabolism
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Summary:Granulocyte/macrophage-colony-stimulating factor (GM-CSF) is an effective growth factor for the blasts of acute myeloblastic leukemia (AML). Radioiodinated Chinese hamster ovary (CHO)-cell derived GM-CSF was prepared using Bolton-Hunter reagent to label free amino groups on the protein. Normal human neutrophils and the blast cells from AML patients were examined for binding. We found that there were fewer receptors of higher affinity on blast cells compared with neutrophils. After brief culture in suspension, receptor number increased and affinity decreased. Experiments provided evidence that GM-CSF from Escherichia coli had a higher affinity for neutrophils (kd = 20 pM) than the CHO-cell derived protein (kd = 500 pM-1 nM). This difference was reflected in the increased effectiveness of the E. coli protein over the CHO protein to stimulate colony formation in both normal bone marrow cells and AML blasts.
ISSN:0887-6924