Effect of new-breviscapine on fibrinolysis and anticoagulation of human vascular endothelial cells

• Published in: Zhōngguó yàolĭ xuébào Vol. 13; no. 3; pp. 239 - 242
• Main Authors: Zhou, Q S, Zhao, Y M, Bai, X, Li, P X, Ruan, C G
• Format: Journal Article
• Language: English
• Published: Beijing Science Press 01.05.1992
• Subjects: Anticoagulants - pharmacology; Biological and medical sciences; Drugs, Chinese Herbal - pharmacology; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Epoprostenol - metabolism; Fibrinolysis - drug effects; Flavonoids; General pharmacology; Humans; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Receptors, Cell Surface - metabolism; Receptors, Thrombin; Thrombin - metabolism; Tissue Plasminogen Activator - metabolism; Umbilical Veins; Human; Blood coagulation; Cell culture; Endothelial cell; Enzyme; Secretion; Arachidonic acid derivatives; Plasminogen activator; Anticoagulant; Compositae; Fibrinolysis; Flavonoid; In vitro; Biological activity; Dicotyledones; Blood vessel; Angiospermae; Plant origin; Spermatophyta; Umbilical vein; Fibrinolytic
• ISSN: 0253-9756

Cultured confluent human umbilical vein endothelial cells were incubated with new-breviscapine (NB), a flavonoid consisting of 4-OH-scutellarin-7-O-glucuronide (C33H30O18) and FeCl3, MgCl2, and CaCl2, which is first extracted from Erigeron breviscapus (Vant) Hand-Mazz in China, 0, 6.25, 12.5, 25, 50, 100, and 1,000 micrograms.ml-1. The releases of tissue-type plasminogen activator (t-PA), and epoprostenol (Epo) from endothelial cells were stimulated by NB, but no significant effect of plasminogen activator inhibitor (PAI) activity was seen. NB 25-1,000 micrograms.ml-1 induced a production of thrombomodulin (TM) within the cells, an expression of TM on the surface of the cells, and a release of TM from the cells. Our data provide a new evidence that NB is a stimulant to fibrinolysis and anticoagulation of endothelial cells.