Microdissection as a means to verify allelic imbalance in tumour biology samples

Allelotypes (TP53, AFM051xd10 and alu-i1) in normal DNA and in DNA from paraffin-embedded tumours of a patient with a p53 germ-line mutation were compared in order to demonstrate LOH. Microdissection was applied in order to overcome difficulties with the interpretation of LOH data from a pelvic recu...

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Bibliographic Details
Published inAnticancer research Vol. 16; no. 1; p. 461
Main Authors Speiser, P, Gharehbaghi-Schnell, E, Schneeberger, C, Eder, S, Zeillinger, R
Format Journal Article
LanguageEnglish
Published Greece 01.01.1996
Subjects
Alleles
Base Sequence
Dissection
DNA - analysis
DNA - genetics
DNA, Neoplasm - analysis
DNA, Neoplasm - genetics
Exons
Gene Deletion
Genes, p53
Heterozygote
Histiocytoma, Benign Fibrous - chemistry
Histiocytoma, Benign Fibrous - genetics
Histiocytoma, Benign Fibrous - pathology
Humans
Lymphocytes - chemistry
Male
Middle Aged
Molecular Sequence Data
Mutation
Paraffin Embedding
Polymorphism, Genetic
Reference Values
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Summary:Allelotypes (TP53, AFM051xd10 and alu-i1) in normal DNA and in DNA from paraffin-embedded tumours of a patient with a p53 germ-line mutation were compared in order to demonstrate LOH. Microdissection was applied in order to overcome difficulties with the interpretation of LOH data from a pelvic recurrence of a primary malignant histiocytoma. Furthermore, a rapid and simple boiling method was developed in order to reduce the loss of DNA usually occurring during traditional methods for DNA extraction. The conclusion drawn is that it is of utmost importance to use highly enriched fractions of tumour cells when performing LOH-studies. It is also shown that a rapid and simple boiling procedure is sufficient to release enough DNA of microdissection-enriched tumour cells for microsatellite analysis by PCR to detect allelic imbalance.
ISSN:0250-7005