Biochemical analyses of murine erythropoietin from plasma and from cloned erythroleukemia cells

The hydrophobicity, ionic charges, degrees of glycosylation, and Western blot patterns of murine plasma erythropoietin and erythropoietin produced by clones of two murine erythroleukemic cell lines (IW32 and NN10) were compared. Erythropoietins from these different sources exhibited a number of simi...

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Published inExperimental hematology Vol. 15; no. 2; p. 171
Main Authors Choppin, J, Egrié, J, Casadevall, N, Lacombe, C, Muller, O, Varet, B
Format Journal Article
LanguageEnglish
Published Netherlands 01.02.1987
Subjects
Animals
Chromatography, Affinity
Chromatography, Ion Exchange
Clone Cells - metabolism
Collodion
Electrophoresis, Polyacrylamide Gel
Erythropoietin - blood
Lectins
Leukemia, Erythroblastic, Acute - metabolism
Leukemia, Erythroblastic, Acute - pathology
Mice
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Summary:The hydrophobicity, ionic charges, degrees of glycosylation, and Western blot patterns of murine plasma erythropoietin and erythropoietin produced by clones of two murine erythroleukemic cell lines (IW32 and NN10) were compared. Erythropoietins from these different sources exhibited a number of similarities in their biochemical properties: identical retention on DEAE Affigel blue column and similar apparent molecular weights (30-36 kDa) in Western blot analysis. However, differences were also observed: heterogeneous binding to different lectin columns and varied retention on a phenyl Sepharose column. The data thus confirm that these murine plasma erythropoietins have biochemical properties in common with previously studied erythropoietins from humans or sheep. The major difference between erythropoietins secreted by erythroleukemic cell lines and other molecules belonging to the same family appears to be related to their glycosylation.
ISSN:0301-472X