Total antioxidant capacity of human seminal plasma

• Published in: Human reproduction (Oxford) Vol. 11; no. 8; pp. 1655 - 1660
• Main Authors: SMITH, R, VANTMAN, D, PONCE, J, ESCOBAR, J, LISSI, E
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.08.1996
• Subjects: Antioxidants; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Humans; Infertility, Male - metabolism; Infertility, Male - physiopathology; Lipid Peroxides - metabolism; Luminescent Measurements; Luminol; Male; Mammalian male genital system; Morphology. Physiology; Reactive Oxygen Species - metabolism; Reference Values; Semen - metabolism; Semen - physiology; Vertebrates: reproduction; Human; Semen; Exploration; Male; Chemiluminescence; Seminal plasma; Antioxidant; Fecundity; Radical scavenger; Free radical; Quantitative analysis
• ISSN: 0268-1161; 1460-2350

Although the importance of seminal plasma in the protection of spermatozoa against reactive oxygen species is well known, only a few studies have investigated its antioxidative properties and the possible relationship between infertility and plasmatic antioxidant defences. The aim of the present study was to assess the status of the total non-enzymatic antioxidant defences of human seminal plasma. Semen samples were obtained from 101 patients consulting for infertility and 15 fertile donors. A total reactive antioxidant potential (TRAP) in seminal plasma was determined by luminol-chemiluminescence. The relationship of seminal TRAP values to lipid peroxidation was also evaluated. Our results show that semen samples from fertile controls show total antioxidant capacity at higher frequency and value than equivalent samples from suspected subfertile men. This fact as well as the inverse relationship observed between antioxidant capacity and lipid peroxidation potential strongly suggest that impaired antioxidant defences may play a role in infertile disorders.