DiI as a marker for cellular transplantation into solid organs

Research in cellular transplantation is frequently compromised by an inability to identify transplanted cells engrafting into orthotopic sites if they exhibit normal morphology and no unique antigenic markers. A method is described for using the fluorescent dye DiI as a marker for cell transplantati...

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Bibliographic Details
Published inBioTechniques Vol. 13; no. 4; p. 580, 582, 584
Main Authors Ledley, F D, Soriano, H E, O'Malley, Jr, B W, Lewis, D, Darlington, G J, Finegold, M
Format Journal Article
LanguageEnglish
Published England 01.10.1992
Subjects
Animals
Carbocyanines
Cell Separation
Cell Transplantation
Flow Cytometry
Fluorescent Dyes
Liver - cytology
Sheep
Staining and Labeling - methods
Thyroid Gland - cytology
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Summary:Research in cellular transplantation is frequently compromised by an inability to identify transplanted cells engrafting into orthotopic sites if they exhibit normal morphology and no unique antigenic markers. A method is described for using the fluorescent dye DiI as a marker for cell transplantation studies. This dye is not metabolized or exchanged between cells in vitro or in vivo and enables identification of engrafted cells by fluorescence microscopy, flow cytometry or fluorescence-activated cell sorting. Applications are described in autologous hepatocellular and thyroid follicular cell transplantation.
ISSN:0736-6205