Control of the growth of leukemic cells (L1210) through manipulation of trace metals

Trace metals have a role in the activity of the enzyme ribonucleotide reductase (RR) which is essential for the synthesis of DNA and the growth of lymphocytes. Manipulation of the intracellular metals of leukemic cells has been proposed for the therapeutic control of cell growth. We studied the effe...

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Bibliographic Details
Published inAnticancer research Vol. 11; no. 4; p. 1561
Main Authors Oblender, M, Carpentieri, U
Format Journal Article
LanguageEnglish
Published Greece 01.07.1991
Subjects
Animals
Cell Division - drug effects
Copper - pharmacology
Deferoxamine - pharmacology
Iron - pharmacology
Leukemia L1210 - metabolism
Leukemia L1210 - pathology
Mice
Ribonucleotide Reductases - metabolism
Trace Elements - pharmacology
Tumor Cells, Cultured
Zinc - pharmacology
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Summary:Trace metals have a role in the activity of the enzyme ribonucleotide reductase (RR) which is essential for the synthesis of DNA and the growth of lymphocytes. Manipulation of the intracellular metals of leukemic cells has been proposed for the therapeutic control of cell growth. We studied the effects of prolonged metal deprivation (Fe, Cu, Zn) on cell growth and RR activity of murine leukemic lymphocytes in culture in metal-depleted media. Intracellular metals, cell growth and RR activity were decreased in related and interdependent ways. A metal-chelator (deferoxamine, DFX) had similar effect. In all cases these effects were reversible by metal supplementation. We conclude that it is possible to control RR activity and growth of leukemic cells in vitro by exposing them to a metal-poor environment (eg. through the action of a chelator). These effects are not permanent, but might be beneficial if integrated with more conventional measures (chemotherapy).
ISSN:0250-7005