Morphological changes in the endocrine pancreas of mice after treatment with streptozotocin combined with cyclophosphamide and neurotropin

The effect of neurotropin (NSP) in combination with streptozotocin (STZ) and cyclophosphamide (CY) on blood glucose and pancreatic histopathology on day 7 and day 14 after the initiation of the treatment was studied in C57Bl/6 male mice. STZ (40 mg/kg) and NSP (1 mg/kg) were applied intraperitoneall...

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Bibliographic Details
Published inCellular and molecular biology Vol. 37; no. 6; p. 575
Main Authors Jablenska, R, Popova, P, Guencheva, G, Duhault, J, Lesourd, M, Petkov, P
Format Journal Article
LanguageEnglish
Published England 1991
Subjects
Animals
Blood Glucose
Cyclophosphamide - administration & dosage
Cyclophosphamide - pharmacology
Drug Combinations
Male
Mice
Mice, Inbred C57BL
Pancreas - drug effects
Pancreas - ultrastructure
Polysaccharides - administration & dosage
Polysaccharides - pharmacology
Streptozocin - administration & dosage
Streptozocin - pharmacology
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Summary:The effect of neurotropin (NSP) in combination with streptozotocin (STZ) and cyclophosphamide (CY) on blood glucose and pancreatic histopathology on day 7 and day 14 after the initiation of the treatment was studied in C57Bl/6 male mice. STZ (40 mg/kg) and NSP (1 mg/kg) were applied intraperitoneally on five consecutive days and CY (150 mg/kg)--twice on day 1 and day 3. In single B cells dilatation of the endoplasmic reticulum was found. On day 7 in proximity to some endocrine cells in the mice treated with STZ, STZ + CY + NSP and STZ + CY macrophages were observed. On day 14 lymphocytic infiltration of the islets was demonstrated only in the groups of mice injected with STZ, STZ + CY while in the group treated with the combination STZ + CY + NSP no infiltration was seen. All experimental groups showed no biochemical evidence for hyperglycemia probably due to the mild destruction of a small number of B cells. The results indicate that NSP might possess a restorative action on insulitis induced by multiple low dose streptozotocin administration in mice.