A simple field method for the purification of Onchocerca ochengi microfilariae from a mixed Onchocerca infection in cattle

Onchocerca ochengi, a bovine parasite, is a suitable model for research on human River Blindness. However, the microfilariae are normally found concomitantly with at least one of the other three bovine Onchocerca species O. dukei, O. gutturosa and O. armillata causing difficulties for the work on th...

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Bibliographic Details
Published inTropical medicine and parasitology Vol. 46; no. 3; p. 201
Main Authors Hagen, H E, Kläger, S L, Ham, P J
Format Journal Article
LanguageEnglish
Published Germany 01.09.1995
Subjects
Animals
Cattle
Cattle Diseases - parasitology
Chromatography, Gel
Microfilariae - classification
Microfilariae - isolation & purification
Onchocerciasis - parasitology
Onchocerciasis - veterinary
Parasitology - methods
Species Specificity
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Summary:Onchocerca ochengi, a bovine parasite, is a suitable model for research on human River Blindness. However, the microfilariae are normally found concomitantly with at least one of the other three bovine Onchocerca species O. dukei, O. gutturosa and O. armillata causing difficulties for the work on the microfilariae. We describe a simple and field applicable method for the separation of living O. ochengi microfilariae from the other Onchocerca species using Sephadex G-25 columns. Elution of mixed populations resulted in the passage of O. gutturosa and/or O. dukei in the initial 1 ml fraction with O. ochengi eluting as an almost 100% pure species in the 4th and 5th fractions.
ISSN:0177-2392