An oocyte-specific methylation imprint center in the mouse U2afbp-rs/U2af1-rs1 gene marks the establishment of allele-specific methylation during preimplantation development

An oocyte-specific methylation imprint mark region, consisting of approximately 200 bp from the mouse imprinted gene U2afbp-rs, was identified within an area containing a CpG island and a short tandem repeat sequence. The oocyte-specific methylation was preserved in fertilized eggs and then expanded...

Full description

Saved in:
Bibliographic Details
Published inGenomics (San Diego, Calif.) Vol. 44; no. 2; pp. 171 - 178
Main Authors SHIBATA, H, UEDA, T, HAYASHIZAKI, Y, KAMIYA, M, YOSHIKI, A, KUSAKABE, M, PLASS, C, HELD, W. A, SUNAHARA, S, KATSUKI, M, MURAMATSU, M
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier 01.09.1997
Subjects
Alleles
Animals
Base Sequence
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
CpG Islands
DNA Methylation
DNA Primers - genetics
Embryonic and Fetal Development - genetics
Embryonic Development - genetics
Female
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genomic Imprinting
In Vitro Techniques
Male
Mice
Oocytes - metabolism
Open Reading Frames
Polymerase Chain Reaction
Pregnancy
Promoter Regions, Genetic
Restriction Mapping
Vertebrata
Embryonic development
Maternal origin
Rodentia
Blastocyst
Genomic imprinting
Vertebrata
Mammalia
Gene
Mouse
DNA
Regulatory sequence
Methylation
Oocyte
Online AccessGet full text

Cover

More Information
Summary:An oocyte-specific methylation imprint mark region, consisting of approximately 200 bp from the mouse imprinted gene U2afbp-rs, was identified within an area containing a CpG island and a short tandem repeat sequence. The oocyte-specific methylation was preserved in fertilized eggs and then expanded on the repressed maternal allele during preimplantation development until the adult methylation pattern was achieved by 12.5 days of embryonic development. These results indicate that the oocyte-specific imprinting mark region acts as a center in establishing the hypermethylated region on the repressed maternal allele. Furthermore, a region that is hypermethylated in both gametes was identified but its hypermethylation was conserved only on the maternal allele during preimplantation development, suggesting that some factor(s) inherited from oocytes may act to maintain hypermethylation on the maternal allele.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1997.4877