Malignant transformation of human prostate epithelial cells by N-nitroso-N-methylurea

We report the malignant transformation of adult human prostate epithelial cells after multiple exposures to the chemical carcinogen N-nitroso-N-methylurea. Such transformants showed morphological alterations and anchorage-independent growth in soft agar and induced carcinomas when transplanted into...

Full description

Saved in:
Bibliographic Details
Published inCancer research (Chicago, Ill.) Vol. 57; no. 4; pp. 576 - 580
Main Authors RHIM, J. S, JIN, S, JUNG, M, THRAVES, P. J, KUETTEL, M. R, WEBBER, M. M, HUKKU, B
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 15.02.1997
Subjects
Adult
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens
Cell Transformation, Neoplastic - chemically induced
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - pathology
Chemical agents
Chromosome Aberrations - chemically induced
Chromosome Disorders
Humans
Karyotyping
Male
Medical sciences
Methylnitrosourea
Mice
Mice, Nude
Neoplasm Proteins - analysis
Prostate - drug effects
Prostate - pathology
ras Proteins - analysis
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - pathology
Tumor Suppressor Protein p53 - analysis
Tumors
Human
Urinary system disease
Prostate disease
Nitrosoureas
Toxicity
Tumorigenicity
Malignant tumor
Carcinogenesis
In vitro
Carcinogen
In vivo
Malignant transformation
Established cell line
Male genital diseases
Prostate
Online AccessGet full text

Cover

More Information
Summary:We report the malignant transformation of adult human prostate epithelial cells after multiple exposures to the chemical carcinogen N-nitroso-N-methylurea. Such transformants showed morphological alterations and anchorage-independent growth in soft agar and induced carcinomas when transplanted into nude mice. No p53 or ras mutations were observed. Stepwise chromosomal changes in the progression to tumorigenicity were observed. Loss of the p arms of chromosome 8 (p10>pter) and chromosome 10 (p10>pter) and gain of the q arm of chromosome 8 (q10>qter) were only observed in the tumor outgrows. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to a chemical carcinogen.
ISSN:0008-5472
1538-7445