Morphological differences in crystals of multiple forms of yeast transketolase

Monocrystals of three individual multiple forms of yeast transketolase (A, B and C) differing in their thermostability have been obtained. Ammonium sulfate was used as a precipitating agent. Crystals of the mentioned forms were found to possess different morphology and stability during storage. Sing...

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Bibliographic Details
Published inBiochemistry international Vol. 26; no. 3; p. 451
Main Authors Kuimov, A N, Konareva, N V, Kochetov, G A
Format Journal Article
LanguageEnglish
Published Australia 01.03.1992
Subjects
Cellulose - analogs & derivatives
Chromatography
Crystallization
Saccharomyces cerevisiae - enzymology
Transketolase - chemistry
Transketolase - metabolism
X-Ray Diffraction
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Summary:Monocrystals of three individual multiple forms of yeast transketolase (A, B and C) differing in their thermostability have been obtained. Ammonium sulfate was used as a precipitating agent. Crystals of the mentioned forms were found to possess different morphology and stability during storage. Single crystals growing from the enzyme form C within 4-7 days were subsequently destroyed. Simultaneously, in the preparation, microcrystals started to grow in a great number. They were found to correspond morphologically to crystals obtained from transketolase A. A possibility of interconversions of the enzyme forms in sequence C---A---B is discussed.
ISSN:0158-5231