Simple methods for estimation of prednisone intake and metabolism

For patients treated with high doses of prednisone (over 40 mg/day) laboratory follow-up is important, particularly when the therapeutic response does not correlate with the dose of prednisone prescribed. Taking advantage of the similarity in structure of prednisone to cortisone and of prednisolone...

Full description

Saved in:
Bibliographic Details
Published inBiomedicine & pharmacotherapy Vol. 42; no. 6; p. 409
Main Authors Maayan, R, Segal, R, Feuerman, E J, Sandbank, M, Kaufman, H
Format Journal Article
LanguageEnglish
Published France 1988
Subjects
Adult
Aged
Chromatography, Thin Layer
Colorimetry
Evaluation Studies as Topic
Female
Fluorometry
Humans
Male
Middle Aged
Prednisone - administration & dosage
Prednisone - metabolism
Prednisone - pharmacokinetics
Radioimmunoassay
Reagent Kits, Diagnostic
Online AccessGet more information

Cover

More Information
Summary:For patients treated with high doses of prednisone (over 40 mg/day) laboratory follow-up is important, particularly when the therapeutic response does not correlate with the dose of prednisone prescribed. Taking advantage of the similarity in structure of prednisone to cortisone and of prednisolone to cortisol we evaluated simple, inexpensive methods for the assessment of prednisone and prednisolone intake and bio-availability. Free urinary prednisolone was estimated by radioimmunoassay for cortisol and found to be linearly correlated with the dose of prednisone administered (r = 0.9310). Levels of free prednisolone were 6.5-11% of the dose of prednisone per day. Free and metabolized prednisone and prednisolone were estimated as 17-OHCS in the 24-hr urine collection. Total, conjugated and free 17-OHCS were linearly correlated with the dose of prednisone, the best correlation being with total 17-OHCS (r = 0.9060). About 40% of the prednisone administered was secreted as total 17-OHCS.
ISSN:0753-3322