Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice
The microbiota confers colonization resistance, which blocks Salmonella gut colonization 1 . As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salm...
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Published in | Nature microbiology Vol. 4; no. 12; pp. 2164 - 2174 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.12.2019
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | The microbiota confers colonization resistance, which blocks
Salmonella
gut colonization
1
. As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted
Salmonella
Typhimurium or
Escherichia coli
gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet.
Salmonella
gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required
Salmonella
’s AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted
Salmonella
gut colonization. Both
E. coli
and
Salmonella
show much higher bile resistance than the microbiota. Correspondingly, competitive
E. coli
can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote
S
. Typhimurium gut infections in mice lacking
E. coli
in their microbiota. This mouse model may be useful for studying pathogen–microbiota–host interactions, the protective effect of
E. coli
, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process.
Short-term exposure to a high-fat diet reduces colonization resistance to
Salmonella
Typhimurium infection in mice and is associated with increase bile salts and plasmid transfer; however,
E. coli
can provide a protective effect under these conditions. |
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ISSN: | 2058-5276 |
DOI: | 10.1038/s41564-019-0568-5 |