Validation and uses of a Y-chromosome STR 10-plex for forensic and paternity laboratories

Y-chromosome short tandem repeats (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of male lineages. The present study validated a Y-STR 10-plex on the ABI PRISM 3100 Genetic Analyzer for use in forensic and paternity labo...

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Bibliographic Details
Published inJournal of forensic sciences Vol. 48; no. 6; p. 1260
Main Authors Johnson, Cassie L, Warren, Joseph H, Giles, Robert C, Staub, Rick W
Format Journal Article
LanguageEnglish
Published United States 01.11.2003
Subjects
Chromosomes, Human, Y
Continental Population Groups - genetics
DNA Fingerprinting - methods
Electrophoresis, Capillary
Female
Gene Frequency
Genetic Variation
Haplotypes
Humans
Male
Paternity
Polymerase Chain Reaction
Sensitivity and Specificity
Tandem Repeat Sequences
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Summary:Y-chromosome short tandem repeats (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of male lineages. The present study validated a Y-STR 10-plex on the ABI PRISM 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid Cellmark. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. This study demonstrated that the 10-plex is sensitive to 0.125 ng of input DNA and that female samples mixed with male samples did not interfere with Y-STR haplotyping. In a sample of 525 males, there were three instances of locus multiplication, two at DYS19 and one at DYS435. Overall, haplotype diversity was 0.996, suggesting that the 10-plex can effectively distinguish among male lineages.
ISSN:0022-1198