Polysaccharide components from the scape of Musa paradisiaca: main structural features of water-soluble polysaccharide component

Polysaccharide components present in the pseudo-stem (scape) of M. paradisiaca were purified from acetone powder of the scape by delignification followed by extraction with aqueous solvents into water soluble polysaccharide (WSP), EDTA-soluble polysaccharide (EDTA-SP), alkali-soluble polysaccharide...

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Bibliographic Details
Published inGlycoconjugate journal Vol. 14; no. 4; pp. 507 - 512
Main Authors Anjaneyalu, Y V, Jagadish, R L, Raju, T S
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.06.1997
Subjects
Aqueous chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Gel
Methylation
Microscopy, Electron
Molecular biology
Molecular Sequence Data
Plant biology
Plants, Medicinal - chemistry
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Solvents
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Summary:Polysaccharide components present in the pseudo-stem (scape) of M. paradisiaca were purified from acetone powder of the scape by delignification followed by extraction with aqueous solvents into water soluble polysaccharide (WSP), EDTA-soluble polysaccharide (EDTA-SP), alkali-soluble polysaccharide (ASP) and alkali-insoluble polysaccharide (AISP) fractions. Sugar compositional analysis showed that WSP and EDTA-SP contained only D-Glc whereas ASP contained D-Glc, L-Ara and D-Xyl in approximately 1:1:10 ratio, respectively, and AISP contained D-Glc, L-Ara and D-Xyl in approximately 10:1:2 ratio, respectively. WSP was further purified by complexation with iso-amylalcohol and characterized by specific rotation, IR spectroscopy, Iodine affinity, ferricyanide number, blue value, hydrolysis with alpha-amylase and glucoamylase, and methylation linkage analysis, and shown to be a amylopectin type alpha-D-glucan.
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ISSN:0282-0080
1573-4986
DOI:10.1023/A:1018563821336