Ultrasound Activatable Microbombs via Co‐Loading CaO2/Perfluorohexane for Cascade Catalytic Biofilm Disruption and Caries Prevention

• Published in: Advanced functional materials Vol. 33; no. 31
• Main Authors: Guo, Jingmei, Liu, Miao‐Deng, Lei, Wenlong, Xu, Yue, Li, Kaifeng, Yu, Jian, Sun, Yun‐Xia, Huang, Cui, Zhang, Xian‐Zheng
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01.08.2023
• Subjects: Biofilms; cascade catalysis; Catalysis; dental caries; Explosions; Free radicals; Hydrogen peroxide; Iron; Materials science; microbombs; oral biofilms; Sterilization; Tannins; Ultrasonic imaging; ultrasonic toothbrushs
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.202213729

Biofilms are the chief culprits of most intractable infections and pose great threat to human health. Developing effective strategies to sweep away the detrimental microbe and stubborn biofilm still remains a challenge. Here, an ultrasound (US) activated microbomb is constructed by encapsulating perfluorohexane (PFH) and iron‐tannin modified calcium peroxide (CaO2‐TA‐Fe) in poly(lactide‐co‐glycolide) (PLGA) vesicles as the reactive oxygen species (ROS) generating reactors for dental biofilm elimination. Upon the US irradiation by ultrasonic toothbrush, the swiftly vaporized PFH can not only promptly explode the PLGA shell to facilitate the generation of H2O2 from CaO2, but also blast the tight biofilm for effective delivery of the lethal hydroxyl free radicals (•OH) caused by degradative Fe3+ from iron‐tannin network, thereby proceeding cascade catalysis eradication of biofilm. As a proof‐of‐concept, this study demonstrates the proposed synergetic paradigm that integrates the US‐triggered explosion with ROS‐induced sterilization for stubborn biofilm elimination, and is a promising tactic for the costly and prevalent caries prevention as well as the biofilm‐associated diseases treatments. Ultrasonic toothbrush activated microbombs are fabricated by encapsulating perfluorohexane (PFH) and iron‐tannin modified calcium peroxide in poly(lactide‐co‐glycolide) microvesicles for biofilm elimination and caries prevention. Upon ultrasound irradiation, the swiftly vaporized PFH can explode to blast biofilm and facilitate the delivery of lethal hydroxyl free radicals via Fenton reaction, thereby proceeding cascade catalysis for bactericide and antibiofilm activities.