Influence of calcium, magnesium and iron ions on the molecular mass of exoproteins during biogranulation

In this study, we performed the qualitative analysis of exoproteins during granule formation in the pres- ence or in the absence of cations. The staining of thin granule cryosections showed that nucleic acids, proteins, polysaccharides and calcium cations were the dominant components of the granules...

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Bibliographic Details
Published inChemical and Process Engineering Vol. 41; no. 4; p. 257
Main Authors Kończak, Beata, Miksch, Korneliusz
Format Journal Article
LanguageEnglish
Published Warsaw Polish Academy of Sciences 01.01.2020
Subjects
Calcium ions
Cations
Electrophoresis
Granular materials
Granulation
Magnesium
Nucleic acids
Polyacrylamide
Polysaccharides
Proteins
Qualitative analysis
Slime
Sodium dodecyl sulfate
Staining
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Summary:In this study, we performed the qualitative analysis of exoproteins during granule formation in the pres- ence or in the absence of cations. The staining of thin granule cryosections showed that nucleic acids, proteins, polysaccharides and calcium cations were the dominant components of the granules. Proteins are the structural components associated with calcium ions. We determined changes in the proteomic profile and tightly bound extracellular polymeric substances (EPS) of the slime. The exopolymeric matrix containing the proteins was extracted using the Dowex resin method. Proteomic profile was analysed by SDS-PAGE method (sodium dodecyl sulphate polyacrylamide gel electrophoresis) using Coomassie blue staining in the samples of the aerobic granule matrix formed in the presence of multivalent cations and compared with that of the aerobic granules cultivated without cations. The results indicate that the granule matrix is predominantly composed of large and complex proteins that are tightly bound within the granular structure. The tightly bound extracellular polymeric substances (TB-EPS) may play a role in improved mechanical stability of aerobic granules. In the supernatant fraction of the sludge, only a small amount of free proteins in the medium molecular mass range was detected. The protein with high molecular mass (? 116 kDa) produced in the reactors with added Ca2+. Ca2+ had a considerable regulatory influence on production of extracellular proteins during aerobic granulation.
ISSN:0208-6425
2300-1925
DOI:10.24425/cpe.2020.136011