A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary

Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analy...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2677; p. 203
Main Authors Soygur, Bikem, Foecke, Mariko H, Gaylord, Eliza A, Fries, Adam, Li, Jing, Arora, Ripla, Laird, Diana J
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Aging
Animals
Female
Fluorescent Antibody Technique
Imaging, Three-Dimensional - methods
Mice
Ovary
Tissue clearing
Ovary
3D analysis
Whole-mount immunofluorescence
Imaging
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Summary:Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3259-8_12