Synthesis of DNA-Templated Silver Nanoclusters and the Characterization of Their Optical Properties and Biological Activity

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2709; p. 299
• Main Authors: Skelly, Elizabeth, Rolband, Lewis A, Beasock, Damian, Afonin, Kirill A
• Format: Journal Article
• Language: English
• Published: United States 2023
• Subjects: Biosensing Techniques - methods; Coloring Agents; DNA - chemistry; DNA Replication; Metal Nanoparticles - chemistry; Silver - chemistry; Silver - pharmacology; Spectrometry, Fluorescence - methods; Fluorescence; Nanocluster; Silver; Antibacterial; AgNC; DNA
• ISSN: 1940-6029
• DOI: 10.1007/978-1-0716-3417-2_21

DNA-templated silver nanoclusters (DNA-AgNCs) are a unique class of bioinorganic nanomaterials. The optical properties and biological activities of DNA-AgNCs are readily modulated by the minor adjustments in the sequence or structure of the templating oligonucleotide. Excitation-emission matrix spectroscopy (EEMS) enables the fluorescence of compounds to be measured in a way that examines the entirety of a material's fluorescent properties. The use of EEMS for the characterization of DNA-AgNCs allows for multiple fluorescence peaks to be readily identified while providing the excitation and emission wavelengths of each signal. To assess the antibacterial and cytotoxic activities of DNA-AgNCs, two separate experimental approaches are used. Assessing the growth of bacteria over time is accomplished by measuring the optical density of the bacterial suspension with 600 nm light, which is directly related to the number of bacteria in suspension. In order to evaluate the DNA-AgNCs for cytotoxic activity, cell viability assays which probe mitochondrial activity were used. Herein, we describe protocols for the characterization of the fluorescent, antibacterial, and cytotoxic activities of DNA-AgNCs using EEM, optical density measurements, and cell viability assays.