1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

• Published in: Nanomedicine (London, England) Vol. 13; no. 12; pp. 1417 - 1432
• Main Authors: Jiang, Jiang, Zhang, Yang, Indra, Arup K, Ganguli-Indra, Gitali, Le, Mai N, Wang, Hongjun, Hollins, Ronald R, Reilly, Debra A, Carlson, Mark A, Gallo, Richard L, Gombart, Adrian F, Xie, Jingwei
• Format: Journal Article
• Language: English
• Published: London Future Medicine Ltd 01.06.2018
• Subjects: Antibiotics; Antimicrobial agents; Bacterial infections; Drug resistance; Gene expression; Hydrogels; Infections; Morphology; Pathogens; Peptides; Vitamin D; Wound healing; United States > US
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm-2018-0011

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.