Identification of Salmonella carriers by amplification of FimA , Stn and InvA genes and bacterial culture methods in fecal samples of buffalo

• Published in: Veterinary research forum Vol. 14; no. 1; pp. 21 - 28
• Main Authors: Ownagh, Abdulghaffar, Etemadi, Navid, Khademi, Peyman, Tajik, Hossein
• Format: Journal Article
• Language: English
• Published: Iran Veterinary Research Forum 2023; Urmia University Press
• Subjects: Animals; Annealing; Bacteria; Cold; Feces; Genes; Livestock; Methods; Original; Polymerase chain reaction; Salmonella; Salmonellosis; Seasons; Azerbaijan; Iran; Asia; Germany; Salmonellosis; Feces; Virulent genes; PCR; Ruminants
• ISSN: 2008-8140; 2322-3618
• DOI: 10.30466/vrf.2022.544308.3312

Salmonellosis is one of the most important bacterial diseases in human and animals. Rapid diagnosis and sub sequence accurate treatment of carriers help reduce the salmonellosis in human and livestock animals. In this study, 420 fecal samples were taken during year 2019 from buffalo in the Urmia, Khoy and Piranshahr regions in west Azerbaijan province, Iran. Samplings were carried out in different seasons. Presence of invasion genes ( , and ) were evaluated by polymerase chain reaction. The bacterial culture and biochemical tests were performed on feces samples for isolation of bacterium ; however, all samples were negative in culture method. PCR findings showed that, 50 (11.90%) fecal samples were positive to the genes. The analysis of results showed that frequency of salmonellosis outbreak in different parts of west Azerbaijan province followed a similar pattern and the incidence of salmonellosis according to forecast in the warm seasons (spring and summer) was more than in cold seasons (autumn and winter). The prevalence of in buffalo's feces based on warm and cold seasons were 32 (64.00%) and 18 (36.00%), respectively. The results showed significant difference between cold and warm season in the prevalence of salmonellosis. Therefore, the application of molecular technics is essential for the prevention and treatment of salmonellosis. The results also showed that specificity of PCR method was better than culture method for detection of in feces sample.