Application of zebrafish microarray on the toxicity mechanism study of bisphenol A

The toxicity mechanism of bisphenol A (BPA) to zebrafish (Danio rerio) was studied in the molecular level,by the method of zebrafish microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Zebrafish embryos were exposed to 0.5, 1.5 and 4.5 mg/L BPA for 8 days...

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Bibliographic Details
Published inHuanjing kexue Vol. 31; no. 3; p. 808
Main Authors Duan, Zheng-hua, Zhu, Lin, Feng, Ming-fen, Bu, Wen-jun, Lam, Siew Hong, Gong, Zhi-yuan
Format Journal Article
LanguageChinese
Published China 01.03.2010
Subjects
Animals
Benzhydryl Compounds
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - metabolism
Endocrine Disruptors - toxicity
Environmental Monitoring - methods
Environmental Pollutants - toxicity
Oligonucleotide Array Sequence Analysis
Phenols - toxicity
Zebrafish - genetics
Zebrafish - metabolism
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Summary:The toxicity mechanism of bisphenol A (BPA) to zebrafish (Danio rerio) was studied in the molecular level,by the method of zebrafish microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Zebrafish embryos were exposed to 0.5, 1.5 and 4.5 mg/L BPA for 8 days since fertilization. The results from microarray and validated by qRT-PCR showed that, 50 specific genes were up-or down-regulated, and dose-responses for them were significant (p < 0.05). This study demonstrates the genotoxicity of BPA. Moreover,according to their function and pathway analysis,it could support the mechanisms for the morphological toxicity and metabolize turbulence observed in prophase study.
ISSN:0250-3301