Optimization of DNA isolation protocol of Aconitum balfourii Stapf.: A rare medicinal herb of Himalayan alpine

Aconitum balfourii Stapf. (Family: Ranunculaceae) is a rare medicinal herb distributed at high altitude (2800-4200 m) of Himalayan region. It is rich in secondary metabolites, especially aconitines, having medicinal properties. Secondary metabolites, mainly polyphenols, cause hinderance in the isola...

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Bibliographic Details
Published inIndian journal of biotechnology Vol. 13; no. 1; pp. 62 - 66
Main Authors Sharma, Eti, Gaur, A K
Format Journal Article
LanguageEnglish
Published 01.01.2014
Subjects
Aconitum
Biotechnology
Contaminants
Deoxyribonucleic acid
In vitro testing
Liquid nitrogen
Medicinal herbs
Metabolites
Optimization
Ranunculaceae
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Summary:Aconitum balfourii Stapf. (Family: Ranunculaceae) is a rare medicinal herb distributed at high altitude (2800-4200 m) of Himalayan region. It is rich in secondary metabolites, especially aconitines, having medicinal properties. Secondary metabolites, mainly polyphenols, cause hinderance in the isolation of good quality DNA from the plant. Therefore, in the present investigation, an attempt was made to determine the total phenolic contents in various parts of in vitro grown plantlets and DNA isolation was optimized from in vitro grown fresh leaves. In the process, 2.5% polyvinyl pyrrolidone was found suitable for obtaining phenolic free DNA. The method also used high salt concentration (5 M NaCl) to remove polysaccharide. In the optimized protocol, DNA isolated with or without liquid nitrogen was comparable in terms of yield and quality. Thus, the suggested protocol provides the contaminant free DNA with good yield that can be used in various molecular biology applications.
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ISSN:0972-5849
0975-0967