Effects of increasing atmospheric CO2 on leaf water δ18O values are small and are attenuated in grasses and amplified in dicotyledonous herbs and legumes when transferred to cellulose δ18O values

• Published in: The New phytologist Vol. 242; no. 5; pp. 1944 - 1956
• Main Authors: Morgner, Eva, Holloway‐Phillips, Meisha, Basler, David, Nelson, Daniel B., Kahmen, Ansgar
• Format: Journal Article
• Language: English
• Published: Lancaster Wiley Subscription Services, Inc 01.06.2024
• Subjects: Amplification; Carbon dioxide; Cellulose; dual‐isotope approach; ecophysiology; elevated CO2; Functional groups; Grasses; Greenhouses; Herbs; Isotope composition; Leaves; Legumes; Oxygen isotopes; Plant species; stable oxygen isotopes; Stomata; Stomatal conductance
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.19713

Summary The oxygen isotope composition of cellulose (δ18O values) has been suggested to contain information on stomatal conductance (gs) responses to rising pCO2. The extent by which pCO2 affects leaf water and cellulose δ18O values (δ18OLW and δ18OC) and the isotope processes that determine pCO2 effects on δ18OLW and δ18OC are, however, unknown. We tested the effects of pCO2 on gs, δ18OLW and δ18OC in a glasshouse experiment, where six plant species were grown under pCO2 ranging from 200 to 500 ppm. Increasing pCO2 caused a decline in gs and an increase in δ18OLW, as expected. Importantly, the effects of pCO2 on gs and δ18OLW were small and pCO2 effects on δ18OLW were not directly transferred to δ18OC but were attenuated in grasses and amplified in dicotyledonous herbs and legumes. This is likely because of functional group‐specific pCO2 effects on the model parameter pxpex. Our study highlights important uncertainties when using δ18OC as a proxy for gs. Specifically, pCO2‐triggered gs effects on δ18OLW and δ18OC are possibly too small to be detected in natural settings and a pCO2 effect on pxpex may render the commonly assumed negative linkage between δ18OC and gs to be incorrect, potentially confounding δ18OC based gs reconstructions.