Polyoxometalates as artificial nucleases: hydrolytic cleavage of DNA promoted by a highly negatively charged ZrIV-substituted Keggin polyanion

A highly negatively charged binuclear ZrIV-substituted Keggin polyoxometalate [{ alpha -PW11O39Zr( mu -OH)(H2O)}2]8- (ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and lin...

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Published inChemical communications (Cambridge, England) Vol. 53; no. 3; pp. 617 - 620
Main Authors Luong, TKN, Govaerts, I, Robben, J, Shestakova, P, Parac-Vogt, T N
Format Journal Article
LanguageEnglish
Published 01.01.2017
Subjects
Cleavage
Deoxyribonucleic acid
Dichroism
Diffusion
Hydrolysis
NMR spectroscopy
Polyoxometallates
Spectroscopy
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Summary:A highly negatively charged binuclear ZrIV-substituted Keggin polyoxometalate [{ alpha -PW11O39Zr( mu -OH)(H2O)}2]8- (ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and linear forms of pUC19 as hydrolysis products. The interaction between ZrK 2 : 2 and DNA was experimentally proven by circular dichroism (CD) spectroscopy and 31P diffusion ordered NMR spectroscopy.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc08555e