Imino‐proton resonances of yeast tRNAPhe studied by two‐dimensional nuclear Overhauser enhancement spectroscopy

• Published in: European journal of biochemistry Vol. 149; no. 3; pp. 649 - 655
• Main Authors: HEERSCHAP, Arend, MELLEMA, Jan‐Remt, JANSSEN, Henny G. J. M., WALTERS, Johannes A. L. I., HAASNOOT, Cornelis A. G., HILBERS, Cornelis W.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 18.06.1985; Blackwell
• Subjects: Applied sciences; Biological and medical sciences; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Imines; Magnetic Resonance Spectroscopy - methods; Molecular biophysics; Nucleic Acid Conformation; Other techniques and industries; Protons; RNA, Fungal - analysis; RNA, Transfer, Amino Acyl - analysis; Saccharomyces cerevisiae - genetics; Spectroscopy : techniques and spectras; Yeast; NMR spectrum; T RNA; Overhauser effect; Two dimension spectrometry
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1111/j.1432-1033.1985.tb08973.x

Application of two‐dimensional nuclear Overhauser enhancement (NOE) spectroscopy to yeast tRNAPhe in H2O solution demonstrates that all imino‐proton resonances, related to the secondary structure, and nearly all imino proton resonances, originating from the tertiary structure, can be assigned efficiently by this method. The results corroborate the assignments of the imino‐proton resonances of this tRNA as established previously by one‐dimensional NOE experiments (only the assignment of base pairs G1· C72 and C2· G71 should be reversed). The advantages of two‐dimensional NOE spectroscopy over one‐dimensional NOE spectroscopy for the assignments of imino‐proton resonances and the structure elucidation of tRNA are illustrated and discussed. Furthermore, the use of non‐exchangeable proton resonances as probes of the molecular structure is explored.