CTLA-4 expressed by human dendritic cells modulates their cytokine secretion and induction of T cell proliferation

• Published in: Journal of Biological Research Vol. 84; no. 1; p. 141
• Main Authors: Laurent, S, Carrega, P, Saverino, D, Piccioli, P, Camoriano, M, Morabito, A, Dozin, B, Fontana, V, Simone, R, Mortara, L, Mingari, M C, Ferlazzo, G, Pistillo, M P
• Format: Journal Article
• Language: English
• Published: Pavia PAGEPress Publications 01.01.2011
• ISSN: 1826-8838; 2284-0230
• DOI: 10.4081/jbr.2011.4651

CTLA-4 is the major negative regulator of T cell responses. We have analyzed the expression of CTLA-4 in human monocytes and monocytederived DCs and the effects of its engagement activity by mature DCs (mDCs). We that CTLA-4 was highly expressed on isolated monocytes, then down-modi the immature DCs (iDCs) and upregulatedv on mDCs. Treatment of mDCs with an agonistic anti-CTLA-4 mAb enhanced secretion of IL10 but reduced secretion of Indian d IL-12, as well as autologous CD4+ T-cellfroliferation in response to stimulation with PPD recall antigenloaded-DCs. Neutralization of IL-10 with an anti-IL-10 antibody partially restored the ability of anti-CTLA-4-treated mDCs to stimulate T cell proliferation in response to PPD. Our data provide the first evidence that CTLA-4 receptor is expressed by human mDCs and exerts immune modulatory effects in these cells.