Identification of Single Nucleotide Polymorphism in rpoB Gene among Mycobacterium tuberculosis Isolates from Pakistan

• Published in: International journal of agriculture and biology Vol. 16; no. 5
• Main Authors: Memona Yasmin Nosheen Mujtaba, Siddiqui, Rubina T
• Format: Journal Article
• Language: English
• Published: Faisalabad AsiaNet Pakistan (Pvt) Ltd 01.10.2014
• Subjects: Mycobacterium tuberculosis
• ISSN: 1560-8530

The emergence of multidrug resistant strains of Mycobacterium tuberculosis is a threat to tuberculosis control programs. The most effective strategy to control the spread of these strains is to use an accurate and rapid drug susceptibility test. Resistance to rifampicin one of the most potent first line anti-TB drugs is found to be associated with mutations in hot-spot" region of RNA polymerase gene (rpoB) are geographically distributed. M. tuberculosis isolates from various regions of Pakistan were collected and mutations were detected by reverse hybridization using in-house line probe assay. The rpoB gene from 45 isolates was cloned and sequenced to validate the results obtained by line probe assay. The most common mutations were found to be in codon 531 (56.45%) followed by codon 526 (9.68%) 516 (4.84%) and 518 (3.22%). Certain silent mutations and several novel mutations outside the hot-spot" region were also found. Copyright 2014 Friends Science Publishers