Identification of Single Nucleotide Polymorphism in rpoB Gene among Mycobacterium tuberculosis Isolates from Pakistan

The emergence of multidrug resistant strains of Mycobacterium tuberculosis is a threat to tuberculosis control programs. The most effective strategy to control the spread of these strains is to use an accurate and rapid drug susceptibility test. Resistance to rifampicin one of the most potent first...

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Bibliographic Details
Published inInternational journal of agriculture and biology Vol. 16; no. 5
Main Authors Memona Yasmin Nosheen Mujtaba, Siddiqui, Rubina T
Format Journal Article
LanguageEnglish
Published Faisalabad AsiaNet Pakistan (Pvt) Ltd 01.10.2014
Subjects
Mycobacterium tuberculosis
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Summary:The emergence of multidrug resistant strains of Mycobacterium tuberculosis is a threat to tuberculosis control programs. The most effective strategy to control the spread of these strains is to use an accurate and rapid drug susceptibility test. Resistance to rifampicin one of the most potent first line anti-TB drugs is found to be associated with mutations in hot-spot" region of RNA polymerase gene (rpoB) are geographically distributed. M. tuberculosis isolates from various regions of Pakistan were collected and mutations were detected by reverse hybridization using in-house line probe assay. The rpoB gene from 45 isolates was cloned and sequenced to validate the results obtained by line probe assay. The most common mutations were found to be in codon 531 (56.45%) followed by codon 526 (9.68%) 516 (4.84%) and 518 (3.22%). Certain silent mutations and several novel mutations outside the hot-spot" region were also found. Copyright 2014 Friends Science Publishers
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ISSN:1560-8530
1814-9596