Multiresidue determination of fluoroquinolone antibiotics in eggs using liquid chromatography-fluorescence-mass spectrometry

An efficient multiresidue method for determination of fluoroquinolone antibiotics in eggs has been developed, in which quantitation and confirmation are achieved simultaneously using fluorescence and MSn. With this method, eight fluoroquinolones were analyzed in fortified egg samples at levels of 10...

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Bibliographic Details
Published inAnalytica chimica acta Vol. 483; no. 1-2; pp. 39 - 49
Main Authors SCHNEIDER, Marilyn J, DONOGHUE, Dan J
Format Conference Proceeding Journal Article
LanguageEnglish
Published Amsterdam Elsevier 25.04.2003
Subjects
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
General pharmacology
Medical sciences
Other chromatographic methods
Pharmacology. Drug treatments
Spectrometric and optical methods
Fluoroquinolones
Egg
Fluorescence
Liquid chromatography
Fluorescence spectrometry
Antibiotic
Residue
Detection limit
Standard deviation
Fluoroquinolone
Animal production
Mass spectrometry
Quantitative analysis
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Summary:An efficient multiresidue method for determination of fluoroquinolone antibiotics in eggs has been developed, in which quantitation and confirmation are achieved simultaneously using fluorescence and MSn. With this method, eight fluoroquinolones were analyzed in fortified egg samples at levels of 10- 100 ng g super(-1). Recoveries for desethylene ciprofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin and difloxacin were generally in the range 60-100%, with excellent relative standard deviations. The limits of quantitation ranged from 0.1 to 2 ng g super(-1) in mixed egg. Confirmation was achieved by monitoring the ratios of two prominent product ions in MS super(2) (desethylene ciprofloxacin) or MS super(3) (all others). The method was found to be applicable to the analysis of egg whites and also egg yolks, with limits of quantitation ranging from 0.1 to 7 ng g super(-1) (egg white) and 0.1-1.5 ng g super(-1) (egg yolk). ENRO-incurred mixed eggs, egg whites and egg yolks were also successfully analyzed using this method.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0003-2670
1873-4324
DOI:10.1016/S0003-2670(02)01378-8