Renal elimination of protein S-100β in pigs with acute encephalopathy

• Published in: Scandinavian journal of clinical and laboratory investigation Vol. 61; no. 3; pp. 217 - 225
• Main Authors: YTREBØ, L. M, NEDREDAL, G. I, KORVALD, C, HOLM NIELSEN, O.-J, INGEBRIGTSEN, T, ROMNER, B, AARBAKKE, J, REVHAUG, A
• Format: Journal Article
• Language: English
• Published: Oslo Scandinavian University Press 01.05.2001
• Subjects: Biological and medical sciences; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Nervous system; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; S100^b protein; S100b protein; Urine; Nervous system diseases; Excretion; Biochemical analysis; Protein S 100B; Acute; Elimination; Exploration; Experimental study; Metabolism; Kidney; Cerebral disorder; Pig; Vertebrata; Liver failure; Mammalia; Animal; Encephalopathy; Clinical biology; Central nervous system disease; Complication; Artiodactyla; Ungulata; Quantitative analysis
• ISSN: 0036-5513; 1502-7686
• DOI: 10.1080/003655101300133658

Protein S-100 beta is an established biochemical marker for cerebral injury in serum. For the further interpretation and possible use of S-100 beta serum measurements in acute hepatic encephalopathy, renal elimination of S-100 beta was measured in pigs with elevated S-100 beta levels due to hepatic encephalopathy. Eighteen female Norwegian Landrace pigs were randomly allocated to either hepatic devascularization (n=13) or sham operation (n=5). Repeated samples from the common carotid artery, right renal vein, and urine were simultaneously drawn for S-100 beta analysis, using the Sangtec100 Liamat immunoassay. In hepatic devascularized pigs, arterial serum levels of S-100 beta increased from 0.96 0.04 mu g/L (mean SEM) at t=0h to 1.74 0.11 mu g/L (mean SEM) at t=5h. Urinary excretion increased simultaneously from 8.48 3.66ng/h (mean SEM) to 20.4 9.54ng/h (mean SEM), while renal arterial-venous fluxes for both kidneys increased 2444 590ng/h (mean SEM). Increased arterial S-100 beta levels in pigs with acute hepatic encephalopathy are not a result of decreased renal elimination. The large difference between the renal arterial-venous S-100 beta concentrations and the urinary excretion of S-100 beta indicate that renal metabolism is the major route of elimination.