Model for prediction of immobilized Acetobacter cell number in calcium alginate gel droplets

The geometry of calcium alginate gel spheres is studied by fractal interpretation for prediction of number of cells to be immobilized. For alginate concentrations from 1 to 5% the simulated results are of 6.65 X 10 super(8), 1.44 X 10 super(8) and 5.27 X 10 super(7) N cell mL super(-1), for respecti...

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Published inBiotechnology techniques Vol. 11; no. 1; pp. 7 - 8
Main Authors MARIGNETTI, N, FUMI, M. D, SILVA, A, BATTISTOTTI, G, POMINI, M, COLAGRANDE, O
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 1997
Subjects
Acetobacter
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Immobilization of organelles and whole cells
Immobilization techniques
Methods. Procedures. Technologies
Acetobacteraceae
Fractal dimension
Prediction
Bacteria
Droplet
Microstructure
Mathematical model
Entrapped microorganism
Modeling
Acetobacter
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Summary:The geometry of calcium alginate gel spheres is studied by fractal interpretation for prediction of number of cells to be immobilized. For alginate concentrations from 1 to 5% the simulated results are of 6.65 X 10 super(8), 1.44 X 10 super(8) and 5.27 X 10 super(7) N cell mL super(-1), for respectively 1.5, 2.5 and 3.5 mm gel sphere diameters. Simulated data are compared with those resulting from literature and particularly with experimental trials where from 10 super(7) to 10 super(8) N cell mL super(-1), in 2% alginate beads with a diameter of 1.5 mm, is able to fulfil mechanical and swelling characteristics of alginate gel beads besides to supply good oxygenation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1007/BF02764441