Effect of Insulin and Insulin-Like Growth Factor 1 (IGF-1) on Activity of Glycogen Synthase in Skeletal Muscles of the Lamprey Lampetra fluviatilis

• Published in: Journal of evolutionary biochemistry and physiology Vol. 39; no. 2; pp. 177 - 183
• Main Authors: Chistyakova, O V, Kuznetsova, L A, Leibush, B N
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01.03.2003
• Subjects: Insulin-like growth factors; Lampetra fluviatilis; Petromyzontidae
• ISSN: 0022-0930; 1608-3202
• DOI: 10.1023/A:1025570315973

Activity of glycogen synthase (GS) in the muscle tissue of the lamprey Lampetra fluviatilis is studied in dynamics of the pre-spawning period as well as under effects of insulin and IGF-1. It is shown that GS exists in the muscles in two forms, the active (I-form) and inactive (D-form), the I-form prevailing during all studied time periods. With approaching the spawning, the GS activity fell 1.5-2 times due to a decrease of the I-form activity. From October to January, both insulin and IGF-1 stimulated GS at concentrations of 10^sup -10^-10^sup -8^ M and 10^sup -9^-10^sup -8^ M, respectively. The maximally effective concentrations (10^sup -9^ M insulin, 10^sup -8^ M IGF-1) produced a 2.5-3-fold rise of the I-form activity of GS at the period from October to December. In January the stimulating effect of these peptides decreased. In March the GS was insensitive both to insulin and to IGF-1. The obtained data indicate participation of insulin and IGF-1 in regulation of glycogen synthesis in lamprey skeletal muscles, the ability of IGF-1 to stimulate the enzyme activity being shown in the lower vertebrates for the first time. It is concluded that IGF-1 takes part in regulation of the carbohydrate metabolism already at early stages of evolution of vertebrates.[PUBLICATION ABSTRACT]