Nanotherapy with hybrid liposomes for colorectal cancer along with apoptosis in vitro and in vivo

• Published in: Anticancer research Vol. 34; no. 9; pp. 4701 - 4708
• Main Authors: Ichihara, Hideaki, Nakagawa, Shinichiro, Matsuoka, Yusuke, Yoshida, Kenshi, Matsumoto, Yoko, Ueoka, Ryuichi
• Format: Journal Article
• Language: English
• Published: Greece 01.09.2014
• Subjects: Animals; Apoptosis - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Dimyristoylphosphatidylcholine - chemistry; Dimyristoylphosphatidylcholine - pharmacology; Disease Models, Animal; Female; Humans; Liposomes - chemistry; Liposomes - pharmacology; Liver Neoplasms - drug therapy; Liver Neoplasms - pathology; Liver Neoplasms - secondary; Mice; Nanomedicine; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Tumor Burden - drug effects; Xenograft Model Antitumor Assays; apoptosis; liver metastasis; Hybrid liposome; colorectal cancer; nanotherapy
• ISSN: 1791-7530

We examined the therapeutic effects of hybrid liposomes (HL) composed of L-α-dimyristylphosphati-dylcholine (DMPC) and polyoxyethylene (25) dodecyl ether (C12(EO)25) on the growth of human colorectal cancer (WiDr) cells in vitro and in vivo. HL composed of 95 mol% DMPC and 5 mol% C12(EO)25 were prepared by the sonication method and their therapeutic effects in xenograft mouse models of colorectal cancer liver metastases were examined in vivo. The inhibitory effects of HL-25 on the growth of WiDr cells along with apoptosis were assessed in vitro. Remarkable inhibitory effects of HL-25 for the liver metastasis of colorectal cancer cells along with apoptosis were revealed on the basis of histological analysis. Prolonged survival was attained for the xenograft mouse model of colorectal cancer after treatment with HL-25 in vivo. Therapeutic effects of HL-25 without any drugs on the liver metastasis of human colorectal cancer were obtained for the first time in vivo.