The cytotoxic protein can induce autophagocytosis in addition to apoptosis in MCF-7 human breast cancer cells

Phagocytic clearance of dying cells is found in many phagocytes. It has been shown that dying cells can be phagocytosed by other phagocytic cells through autophagic or apoptotic cellular death. To date, whether cancer cells have such phagocytic activity has not been studied. In this study, our data...

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Published inIn vivo (Athens) Vol. 26; no. 3; pp. 403 - 409
Main Authors Yiang, Giou-Teng, Yu, Yung-Luen, Chou, Pei-Lun, Tsai, Hsiu-Feng, Chen, Li-Ann, Chen, Yen Hsieh, Su, Kuo-Jung, Wang, Jaang-Jiun, Bau, Da-Tian, Wei, Chyou-Wei
Format Journal Article
LanguageEnglish
Published Greece 01.05.2012
Subjects
Amphibian Proteins - pharmacology
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Autophagy - drug effects
Breast Neoplasms
Caspase Inhibitors
Caspases - metabolism
Cell Line, Tumor
Cell Survival - drug effects
Endoribonucleases - pharmacology
Female
Humans
Single-Cell Analysis
Time-Lapse Imaging
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Summary:Phagocytic clearance of dying cells is found in many phagocytes. It has been shown that dying cells can be phagocytosed by other phagocytic cells through autophagic or apoptotic cellular death. To date, whether cancer cells have such phagocytic activity has not been studied. In this study, our data shows that RC-RNase can trigger cell death in human breast cancer MCF-7 cells through the apoptotic pathway. Interestingly, when treated with cytotoxic protein, the remaining MCF-7 cells can phagocytose the dying MCF-7 cells via autophagocytic activity, demonstrated directly by real-time image observation and electron microscopy analysis. To sum up, this study demonstrates for the first time that RC-RNase can trigger apoptosis and autophagocytosis in MCF-7 cancer cells.
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ISSN:1791-7549