Evaluation of susceptibility to organophosphates in populations of Aedes aegypti in La Guajira, Colombia

• Published in: Biomedica : revista del Instituto Nacional de Salud Vol. 43; no. 2; p. 296
• Main Authors: Maestre-Serrano, Ronald, Flórez-Rivadeneira, Zulibeth, Castro-Camacho, Juan Miguel, Ochoa-Bohórquez, Linda, Gómez-Camargo, Doris, Pareja-Loaiza, Paula, Ponce-García, Gustavo, Flores, Adriana E
• Format: Journal Article
• Language: English
• Published: Colombia 30.06.2023
• Subjects: Aedes; Animals; Colombia; Mosquito Vectors; Organophosphates; Temefos; United States; Colombia; United States; organophosphate; Colombia; malathion; Aedes aegypti; temefos; insecticide resistance; insecticides
• ISSN: 2590-7379
• DOI: 10.7705/biomedica.6677

Introduction. Dengue is a public health problem in La Guajira region. Control has focused on the vector using insecticides, including organophosphates. Objective. To evaluate the state of susceptibility to organophosphates insecticides in fifteen Aedes aegypti (L.) populations in La Guajira, Colombia. Materials and methods. We collected samples of third-instar larvae and adult mosquitoes of Ae. aegypti in the municipalities of Albania, Barrancas, Dibulla, Distracción, El Molino, Fonseca, Hatonuevo, La Jagua del Pilar, Maicao, Manaure, Riohacha, San Juan del Cesar, Uribia, Urumita, Villanueva. Bioassays for temefos, malathion, and pirimiphos-methyl were carried out following the methodology of the World Health Organization, and the bottle technique using the guidance of the Centers for Disease Control and Prevention. Susceptibility to temefos was determined through the resistance ratio between lethal concentration 50 and lethal concentration 95; for the compounds temefos, malathion and pirimiphos-methyl, susceptibility was calculated using diagnostic dose and diagnostic time in the populations evaluated. Rockefeller susceptible strain was used as a control. Results: All evaluated populations of Ae. aegypti from La Guajira were found to be susceptible to temefos (ratio resistance to CL50<5.0; ratio resistance to CL95<5.0; 98 - 100 % mortality); pirimiphosmethyl (99 - 100 % mortality), and malathion (100 % mortality). Conclusion. Based on the results, the use of temefos, malathion, and pirimiphosmethyl is feasible for the control of Ae. aegypti in the evaluated populations.