The incorporation of extracellular matrix proteins in protein polymer hydrogels to improve encapsulated beta-cell function

Biomaterial encapsulation of islets has been proposed to improve the long-term success of islet transplantation by recreating a suitable microenvironment and enhancing cell-matrix interactions that affect cellular function. Protein polymer hydrogels previously showed promise as a biocompatible scaff...

Full description

Saved in:
Bibliographic Details
Published inAnnals of clinical and laboratory science Vol. 43; no. 2; p. 111
Main Authors Beenken-Rothkopf, Liese N, Karfeld-Sulzer, Lindsay S, Davis, Nicolynn E, Forster, Ryan, Barron, Annelise E, Fontaine, Magali J
Format Journal Article
LanguageEnglish
Published United States 2013
Subjects
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Animals
Biocompatible Materials - metabolism
Cell Line
Cell Survival - drug effects
Cellular Microenvironment - physiology
Chromatography, Affinity
Collagen
Extracellular Matrix Proteins - pharmacology
Fibronectins
Hydrogels - metabolism
Insulin-Secreting Cells - drug effects
Insulin-Secreting Cells - physiology
Islets of Langerhans Transplantation - methods
Laminin
Mice
Molecular Sequence Data
Polymers - pharmacology
Rheology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
extracellular matrix proteins
hydrogel
islet transplantation
Online AccessGet full text

Cover

More Information
Summary:Biomaterial encapsulation of islets has been proposed to improve the long-term success of islet transplantation by recreating a suitable microenvironment and enhancing cell-matrix interactions that affect cellular function. Protein polymer hydrogels previously showed promise as a biocompatible scaffold by maintaining high cell viability. Here, enzymatically-crosslinked protein polymers were used to investigate the effects of varying scaffold properties and of introducing ECM proteins on the viability and function of encapsulated MIN6 β-cells. Chemical and mechanical properties of the hydrogel were modified by altering the protein concentrations while collagen IV, fibronectin, and laminin were incorporated to reestablish cell-matrix interactions lost during cell isolation. Rheology indicated all hydrogels formed quickly, resulting in robust, elastic hydrogels with Young's moduli similar to soft tissue. All hydrogels tested supported both high MIN6 β-cell viability and function and have the potential to serve as an encapsulation platform for islet cell delivery in vivo.
ISSN:1550-8080