Research on error reduction of path change of liquid samples based on near infrared trans-reflective spectra measurement

• Published in: Guang pu xue yu guang pu fen xi Vol. 34; no. 10; p. 2863
• Main Authors: Wang, Ya-Hong, Dong, Da-Ming, Zhou, Ping, Zheng, Wen-Gang, Ye, Song, Wang, Wen-Zhong
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2014
• ISSN: 1000-0593
• DOI: 10.3964/j.issn.1000-0593(2014)10-2863-05

Based on sucrose solution as the research object, this paper measured the trans-reflective spectrum of sucrose solution of different concentration by the technique of near infrared spectrum in three optical path (4, 5, 6 mm). Five kinds of pretreatment method (vector normalization, baseline offset correction, multiplicative scatter correction, standard normal variate transformation, a derivative) were used to eliminate the influence of the optical path difference, and to establish model of the calibration set in combination with the PLS (Partial Least Squares)method. Five kinds of pretreatment method could restrain the inter ference of light path in varying degrees. Compared with the PLS model of original spectra, the model of multiple scattering correction combined with PLS method is the optimal model. The results of quantitative analysis of original spectra: the number of principal component PC= 6, the determination coefficient R2 = 0.891 278, the determination coefficient of cross validation R2CV = 0.888 3