Hematocrit-independent recovery is a key for bioanalysis using volumetric absorptive microsampling devices, Mitra

A novel microsampling device, Mitra(TM), was evaluated for bioanalysis of E6005 and its O-desmethylated metabolite in human whole blood using an UPLC-MS. A constant volume of blood samples was absorbed onto the tip of Mitra, the analytes were extracted by various solvents and then detected by UPLC-M...

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Bibliographic Details
Published inBioanalysis Vol. 7; no. 15; p. 1821
Main Authors Mano, Yuji, Kita, Kenji, Kusano, Kazutomi
Format Journal Article
LanguageEnglish
Published England 01.01.2015
Subjects
Blood Specimen Collection - instrumentation
Dried Blood Spot Testing - instrumentation
Hematocrit - methods
Humans
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Summary:A novel microsampling device, Mitra(TM), was evaluated for bioanalysis of E6005 and its O-desmethylated metabolite in human whole blood using an UPLC-MS. A constant volume of blood samples was absorbed onto the tip of Mitra, the analytes were extracted by various solvents and then detected by UPLC-MS. Recovery of the analytes was high in acetonitrile-water (1:1, v/v) but was dependent on hematocrit (Hct) without sonication process, which led to biased accuracy at low and high Hcts. Inclusion of sonication process in extraction improved recovery at high Hct to yield acceptable accuracy across Hcts. Optimization of extraction process to achieve high recovery regardless of Hct is critical in accurate bioanalysis via Mitra.
ISSN:1757-6199
DOI:10.4155/bio.15.111