Transplantation with cultured stem cells derived from the human amniotic membrane for corneal alkali burns: an experimental study

Amniotic membranes (AM) have been used in a wide range of clinical applications. We successfully extracted mesenchymal stem cells (MSCs) from human AM, but little is known about the use and efficacy of human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) for the treatment of alkali bur...

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Published inAnnals of clinical and laboratory science Vol. 44; no. 1; pp. 73 - 81
Main Authors Zeng, Wei, Li, Yanwei, Zeng, Guangwei, Yang, Bo, Zhu, Yu
Format Journal Article
LanguageEnglish
Published United States 2014
Subjects
Alkalies
Amnion - cytology
Animals
Burns - pathology
Burns - therapy
Cell Movement
Cell Proliferation
Cells, Cultured
Corneal Neovascularization - pathology
Corneal Neovascularization - therapy
Corneal Opacity
Dextrans - metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Epithelium, Corneal - pathology
Female
Humans
Inflammation - pathology
Magnetite Nanoparticles
Male
Rabbits
Stem Cell Transplantation
Stem Cells - cytology
Tumor Necrosis Factor-alpha - metabolism
Wound Healing
Amniotic membrane
alkali burns
mesenchymal stem cells
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Summary:Amniotic membranes (AM) have been used in a wide range of clinical applications. We successfully extracted mesenchymal stem cells (MSCs) from human AM, but little is known about the use and efficacy of human amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) for the treatment of alkali burns. We utilized hAM-dMSCs transplantation, AM grafting, and their combined use in the treatment of alkali burns. An experimental model in rabbits was devised to analyze the use of these techniques with immunocytochemistry and ELISA. The survival and migration of hAM-dMSCs labeled by SPION in the host were assessed with Prussian blue staining. Compared with the control group, the treated groups demonstrated faster reconstruction of the corneal epithelium, and lower levels of corneal opacification and neovascularization within corneal alkali burns. Furthermore, dark blue-stained particles were detected in the limbus corneae at day 28. These results demonstrated the ability of hAM-dMSCs to enhance epithelial healing and reduce corneal opacification and neovascularization in corneal alkali wounds.
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ISSN:1550-8080