G894T (NOS3) and G1958A (MTHFD1) gene polymorphisms and risk of ischemic heart disease in Yucatan, Mexico

• Published in: Clinica e investigacion en arteriosclerosis : publicacion oficial de la Sociedad Espanola de Arteriosclerosis Vol. 27; no. 2; p. 64
• Main Authors: García-González, Igrid, Solís-Cárdenas, Alberto de Jesús, Flores-Ocampo, Jorge A, Alejos-Mex, Ricardo, Herrera-Sánchez, Luis Fernando, González-Herrera, Lizbeth Josefina
• Format: Journal Article
• Language: Spanish
• Published: Spain 01.03.2015
• Subjects: Adult; Alleles; Aminohydrolases - genetics; Angina Pectoris - genetics; Angina Pectoris - physiopathology; Case-Control Studies; Coronary Disease - genetics; Coronary Disease - physiopathology; Female; Formate-Tetrahydrofolate Ligase - genetics; Genetic Predisposition to Disease; Genotype; Humans; Logistic Models; Male; Methylenetetrahydrofolate Dehydrogenase (NADP) - genetics; Mexico - epidemiology; Middle Aged; Multienzyme Complexes - genetics; Myocardial Ischemia - genetics; Myocardial Ischemia - physiopathology; Nitric Oxide Synthase Type III - genetics; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Risk Factors; Ischemic heart disease; Genotipo; Genotype, Polymorphism, NOS3; MTHFD1; NOS3; Polimorfismo; Cardiopatía isquémica
• ISSN: 1578-1879
• DOI: 10.1016/j.arteri.2014.07.002

Cardiovascular medicine is focused on the search for genetic risk markers with predictive and/or prognostic value. Among the genetic variants of interest are G894T endothelial nitric oxide synthase and G1958A methylenetetrahydrofolate dehydrogenase1 gene polymorphisms. The aim of this study was to determine the possible association between these polymorphisms and ischemic heart disease in patients from Southern of Mexico (Yucatán). Case-control study matched by age, sex and origin was designed. We studied 98 patients with coronary disease and 101 controls. Participants were evaluated for the usual risk factors. The polymorphisms were identified using the polymerase chain reaction/restriction fragment length polymorphism analysis. Informed consent was obtained from all participants. The G894T and G1958A polymorphisms were not associated with ischemic heart disease, however, the TT genotype (G894T) was associated with the angina (OR=10.2; 95%CI, 1.51-68.8; p=0.025). The genotype GT (G894T) was the most frequent in patients with family history of coronary artery disease. Multiple logistic regression analysis identified smoking (OR=5.21; 95%CI, 2.1-12.9; p=0.000), hypertension (OR=3.54; 95%CI, 1.47-8.56; p=0.005) and obesity (OR=1.16; 95%CI, 1.1-1.27; p=0.001) as risk factors predicting the ischemic heart disease. The G894T and G1958A polymorphisms showed not association with ischemic heart disease. However, homozygosis for the 894T allele (NOS3) confers at risk to develop angina on Yucatán.