Oxidative stress and antioxidant defense in patients with chronic hepatitis B

• Published in: Clinical laboratory (Heidelberg) Vol. 58; no. 3-4; p. 273
• Main Authors: Tasdelen Fisgin, Nuriye, Aydin, Birsen Kilicoglu, Sarikaya, Hanife, Tanyel, Esra, Esen, Saban, Sunbul, Mustafa, Leblebicioglu, Hakan
• Format: Journal Article
• Language: English
• Published: Germany 2012
• Subjects: Adult; Ascorbic Acid - blood; Carrier State - metabolism; Carrier State - virology; Case-Control Studies; Female; Glutathione - blood; Glutathione Peroxidase - blood; Hepatitis B, Chronic - metabolism; Hepatitis B, Chronic - virology; Humans; Lipid Peroxidation; Male; Middle Aged; Oxidative Stress; Protein Carbonylation; Viral Load; Vitamin E - blood
• ISSN: 1433-6510

Oxidative stress is defined as a disturbance of balance between free radicals and antioxidant defense system. This study investigated oxidative stress in patients with chronic hepatitis B. Sixty nine patients with chronic hepatitis B admitted to the Department of the Infectious Diseases and Clinical Microbiology of Medical Faculty of Ondokuz Mayis University were enrolled into study. Twenty healthy persons were included as a control group. The study group was divided into three groups: healthy controls (group 1), chronic hepatitis B (group 2), and inactive hepatitis B carriers (group 3). Antioxidant status of plasma, including glutathione, glutathione peroxidase, vitamin E, and vitamin C levels were measured. Carbonyl and lipid peroxidation levels were measured as parameters of oxidative stress. Glutathione, glutathione peroxidase, vitamin E, and vitamin C levels were found to be significantly decreased in the chronic hepatitis B group when compared with the control group (9.5 vs. 13.8, p < 0.05; 22.98 vs. 32.4, p < 0.05; 15.1 vs. 16.4, p < 0.05; 12.9 vs. 18.4, p < 0.05, respectively). Carbonyl and lipid peroxidation levels were significantly increased in the chronic hepatitis B group compared to controls (0.7 vs. 0.5, p < 0.05; 2 vs. 0.7, p < 0.05, respectively). However, whereas the glutathione and carbonyl level correlation with HBV DNA levels were mild to moderate (glutathione vs. HBV DNA, r:-0.288, p < 0.05; carbonyl vs. HBV DNA, r:0.317, p < 0.05), the lipid peroxidation levels were strongly related with HBV DNA levels in chronic hepatitis B (r:0.545, p < 0.05). Oxidative stress was significantly increased in hepatitis B patients. Consequently, decreases were seen at the level of protective antioxidative parameters in the blood of these patients.