Biochemical markers assisted screening of Fusarium wilt resistant Musa paradisiaca (L.) cv. puttabale micropropagated clones

An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction...

Full description

Saved in:
Bibliographic Details
Published inIndian journal of experimental biology Vol. 51; no. 7; pp. 531 - 542
Main Authors Venkatesh, Krishna, V, Kumar, K Girish, Pradeepa, K, Kumar, S R Santosh, Kumar, R Shashi
Format Journal Article
LanguageEnglish
Published India 01.07.2013
Subjects
Benzyl Compounds
Biomarkers - analysis
Cells, Cultured
Fusarium - genetics
Fusarium - pathogenicity
Host-Pathogen Interactions
Kinetin - pharmacology
Musa - drug effects
Musa - genetics
Musa - microbiology
Phenylurea Compounds - pharmacology
Plant Diseases - genetics
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Shoots - drug effects
Plant Shoots - genetics
Plant Shoots - microbiology
Purines
Thiadiazoles - pharmacology
Online AccessGet full text

Cover

More Information
Summary:An efficient protocol was standardized for screening of panama wilt resistant Musa paradisiaca cv. Puttabale clones, an endemic cultivar of Karnataka, India. The synergistic effect of 6-benzyleaminopurine (2 to 6 mg/L) and thidiazuron (0.1 to 0.5 mg/L) on MS medium provoked multiple shoot induction from the excised meristem. An average of 30.10 +/- 5.95 shoots was produced per propagule at 4 mg/L 6-benzyleaminopurine and 0.3 mg/L thidiazuron concentrations. Elongation of shoots observed on 5 mg/L BAP augmented medium with a mean length of 8.38 +/- 0.30 shoots per propagule. For screening of disease resistant clones, multiple shoot buds were mutated with 0.4% ethyl-methane-sulfonate and cultured on MS medium supplemented with Fusarium oxysporum f. sp. cubense (FOC) culture filtrate (5-15%). Two month old co-cultivated secondary hardened plants were used for screening of disease resistance against FOC by the determination of biochemical markers such as total phenol, phenylalanine ammonia lyase, oxidative enzymes like peroxidase, polyphenol oxidase, catalase and PR-proteins like chitinase, beta-1-3 glucanase activities. The mutated clones of M. paradisiaca cv. Puttabale cultured on FOC culture filtrate showed significant increase in the levels of biochemical markers as an indicative of acquiring disease resistant characteristics to FOC wilt.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0019-5189