Phytochemical analysis and antioxidant capacity of Tabernaemontana catharinensis A. DC. Fruits and branches

• Published in: Anais da Academia Brasileira de Ciências Vol. 86; no. 2; pp. 881 - 888
• Main Authors: Piana, Mariana, Boligon, Aline A, Brum, Thiele F DE, Zadra, Marina, Belke, Bianca V, Froeder, Amanda L F, Frohlich, Janaína K, Nunes, Letícia T, Pappis, Lauren, Boligon, Alexandra A, Athayde, Margareth L
• Format: Journal Article
• Language: English
• Published: Brazil 01.06.2014
• ISSN: 1678-2690
• DOI: 10.1590/0001-3765201420120020

The antioxidant capacity of the crude extract and fractions of Tabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.