Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

• Published in: Anticancer research Vol. 35; no. 5; pp. 2817 - 2822
• Main Authors: Oshima, Celina T F, Ribeiro, Daniel A, Gomes, Thiago S, Adios, Priscila C, Egami, Mizue I, Segreto, Helena R C
• Format: Journal Article
• Language: English
• Published: Greece 01.05.2015
• Subjects: Amifostine - administration & dosage; Animals; Caspase 3 - biosynthesis; Colon - metabolism; Colon - pathology; Colon - radiation effects; Gene Expression Regulation, Neoplastic - radiation effects; Humans; Mice; Radiation-Protective Agents - administration & dosage; Whole-Body Irradiation; mouse; apoptosis; radiation; Amifostine
• ISSN: 1791-7530

The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+)group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.