Effect of borate polymer layers on measurement of glucose concentration by SPR

• Published in: Guang pu xue yu guang pu fen xi Vol. 33; no. 1; p. 142
• Main Authors: Li, Da-Chao, Yang, Jia, Wu, Peng, Zhu, Rui, Wang, Bo, Lin, Yuan, Xu, Ke-Xin
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2013
• Subjects: Acrylic Resins - chemistry; Adsorption; Blood Glucose - analysis; Borates - chemistry; Boronic Acids - chemistry; Glucose - analysis; Glucose - chemistry; Humans; Polymers - chemistry; Surface Plasmon Resonance - methods
• ISSN: 1000-0593
• DOI: 10.3964/j.issn.1000-0593(2013)01-0142-05

A new borate polymer PAA-ran-PAAPBA that can specifically adsorb glucose was introduced in the glucose measurement based on surface plasmon resonance, and the high-precision specific detection of glucose concentration was realized. Six layers and twelve layers of borate polymer were respectively bound onto the SPR sensors through the layer-by-layer self-assembly binding method, and the effect of different layers of borate polymer on the glucose surface plasmon resonance measurement was studied. The experiment was conducted in the concentration range of 1-10 mg x dL(-1) (interval delta = 1 mg x dL(-1)), 10-100 mg x dL(-1) (interval delta = 10 mg x dL(-1)), and 100-1 000 mg x dL(-1) (interval delta = 100 mg x dL(-1)), experiment data was fitted by quadric curve and the fitting degree of refractive index difference deltaRU and glucose concentration was obtained. Results showed that the 12-layer-polymer sensor was better than the 6-layer-polymer sensor in the first two smaller ranges, and the measuring result was