Antibacterial, antifungal and enzymatic activities of azithromycin-heavy metal complexes: Newly synthesized and characterized

• Published in: Pakistan journal of pharmaceutical sciences Vol. 34; no. 3(Supplementary); pp. 1149 - 1156
• Main Authors: Shamim, Sana, Begum, Irshad, Gul, Wajiha, Quds, Tehseen, Imran, Muhammad, Shah, Erum, Jahan, Noor
• Format: Journal Article
• Language: English
• Published: Pakistan 01.05.2021
• Subjects: Anti-Bacterial Agents - pharmacology; Antifungal Agents - pharmacology; Arsenic Trioxide - chemistry; Arsenic Trioxide - pharmacology; Azithromycin - analogs & derivatives; Azithromycin - chemistry; Azithromycin - pharmacology; Bacillus subtilis - drug effects; Candida albicans - drug effects; Carbonates - chemistry; Carbonates - pharmacology; Chymotrypsin - metabolism; Citrobacter - drug effects; Coordination Complexes - chemistry; Coordination Complexes - pharmacology; Disk Diffusion Antimicrobial Tests; Enzyme Assays; Escherichia coli - drug effects; Klebsiella pneumoniae - drug effects; Lead - chemistry; Lead - pharmacology; Micrococcus luteus - drug effects; Proteus mirabilis - drug effects; Pseudomonas aeruginosa - drug effects; Salmonella typhi - drug effects; Shigella flexneri - drug effects; Silver Compounds - chemistry; Silver Compounds - pharmacology; Staphylococcus aureus - drug effects; Streptococcus - drug effects; Urease - metabolism
• ISSN: 1011-601X
• DOI: 10.36721/PJPS.2021.34.3.SUP.1149-1156.1

As part of our continuous research to understand the interaction mechanism of drug and metallo-elements, heavy metal complexes of azithromycin (AZI) were synthesized with arsenic oxide, lead carbonate and silver chloride salts in molar ratio of 2: 1 (L: M). Synthesized heavy metal complexes have shown good percent yield and characterized through spectroscopic parameters including UV-Visible, TLC, FT-IR, NMR and elemental analysis (CHN). Spectroscopic characterization reveals the binding of ligand AZI with heavy metals in bi-dentate manner involving the hydroxide and 9a-NCH group of the aglycone ring of AZI. These newly synthesized heavy metal complexes were evaluated for their antimicrobial response against selected gram positive and gram negative organisms and antifungal species. It was noted that all newly synthesized complexes exhibits increased activity against B.subtilus whereas, AZI itself didn't show any activity, while synthesized complexes have low to moderate response against all the studied organisms. Complex A-M12 possess greater enzymatic response against both urease and alpha chymotrypsin among all the studied complexes. Results obtained were then statistically analyzed through one way ANOVA and Dunnett's test by using SPSS version 20.0 suggesting the significant response of complexes against selected organisms.