Downregulation of SIRT6 and NMNAT2 is associated with proliferative diabetic retinopathy

• Published in: Molecular vision Vol. 29; p. 160
• Main Authors: Chen, Hui, Zhang, Xiongze, Liao, Nanying, Ji, Yuying, Mi, Lan, Gan, Yuhong, Su, Yongyue, Wen, Feng
• Format: Journal Article
• Language: English
• Published: United States 2023
• Subjects: Diabetes Mellitus, Type 2 - complications; Diabetic Retinopathy - genetics; Diabetic Retinopathy - metabolism; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Humans; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Leukocytes, Mononuclear - metabolism; Nicotinamide-Nucleotide Adenylyltransferase - genetics; Nicotinamide-Nucleotide Adenylyltransferase - metabolism; Sirtuins - genetics; Sirtuins - metabolism; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1090-0535; 1090-0535

To determine the expression levels of and in diabetic retinopathy (DR). We obtained peripheral blood mononuclear cells (PBMCs) and vitreous samples from 77 patients with type 2 diabetes mellitus: 52 with DR and 25 without DR, and 27 healthy control subjects. Western blot analysis and qRT-PCR were performed to evaluate the expression of and in their PBMCs. The levels of , , and in the vitreous fluid were determined by ELISA. Immunohistochemistry was performed to detect the expression of and 2 in proliferative DR (PDR) and the control subjects. The expression of and was markedly downregulated in DR patients, which was negatively correlated with the increased expression of and . Additionally, we observed decreased expression of and in the fibrovascular membranes of PDR patients. The downregulated expression of and in PDR patients reveals a potential pathogenic association; more extended studies could verify them as potential therapeutic targets.